P-22: Effect of Bovine Cumulus Cell Co-Cultureon Maturation of Buffalo (Bubalus Bubalis)Oocyte

P-87: Effects of Bovine Cumulus Cell Co-Cul tire on Post Fertilization Development of Buffalo (Bubalus bubalis) Oocyte

Background: There is a considerable numbers of buffalo (Bubalus bubalis) in Iran which have an important role in rural life. The reproductive performance of buffalo is lower than cow. Co-culture with somatice cells improved the maturation rate of most of the species. The aim of the present study was to evaluate the effect of bovine cumulus co-culture on post fertilization development of buffalo...

Effect of Leptin on In Vitro Nuclear Maturation and Apoptosis of Buffalo (Bubalus bubalis) Oocyte

Effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (Bubalus bubalis) oocytes in vitro

AIM The aim was to assess the effects of fetal bovine serum (FBS) and estrus buffalo serum (EBS) on in vitro maturation rate of oocytes in buffalo. MATERIALS AND METHODS Maturation rate of oocytes was assessed in two maturation media supplemented with 20% FBS and EBS. Oocytes maturation rate was evaluated on the basis of cumulus cell expansion and extrusion of polar body after 24 h of in vitr...

P-110: Effect of Increasing Amount of Oocyte Secreted Factors on Cumulus Expansion of Bovine Cumulus-Oocyte Complexes

Background: In vitro maturation is a good method to decrease cancer risk of superovulation by gonadotropin hormones. A paracrine effect of oocyte secretions on oocyte developmental competence is under investigation. Apart from oocyte maturation, ovulation in vivo requires a precise control of extracellular matrix modification. Cumulus cells secrete hyaluronan to form a muco-elastic extracellula...

the effect of e-64 on the developmental competence of sheep cocs during in vitro maturation

in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.

9-cis Retinoic acid inhibits cumulus cell apoptosis during the maturation of bovine cumulus-oocyte-complexes.

Cumulus cell (CC) apoptosis is inversely correlated with embryonic development in vitro. Therefore, inhibition of CC apoptosis is important for proper embryonic development and quality. Retinoic acids (all-transRA and 9-cisRA) are natural components of retinoids, and 9-cisRA is the physiologically active metabolite of retinoic acid in vitro. During in vitro maturation, 9-cisRA enhances oocyte c...